HYPSIBOAS ALBOPUNCTATUS PDF

About CORE Blog Contact us Location of Repository Hypsiboas albopunctatus Anura, Hylidae populations of urban and rural environments differ with respect to vocalization, morphology and population size? In recent years, population declines of amphibians have been observed in different parts of the world. The process of fragmentation and destruction of habitats is considered the most threat to this diversity. Urbanization reduces species richness at assemblage of amphibian reproduction. Due to amphibians require a modest area for survival, some species can thrive in urban fragments. But small population sizes and isolation can cause the loss of genetic diversity and the extinction local.

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Comp Cytogenet. Published online Aug 8. This article has been cited by other articles in PMC. Reverse hybridisation was also carried out in the investigation. The B probe exclusively painted the supernumerary, not hybridising any other chromosomes in H.

This result might be an indication that differences exist between the repetitive sequences of A and B chromosomes of H. Although our results could suggest an interespecific origin of the B at first sight, further analysis on its repetitive sequences is still necessary. Nevertheless, the accumulation of repetitive sequences, detected in another species, even though closely related, remains an intriguing question.

With exception of dispensability for normal growth and development, many of the B characteristics, for example, numerical variability within and between individuals, smaller size than chromosomes from the A complement, heterochromatic nature, abnormal segregation at cell divisions, and non-mendelian inheritance, are not universal, indicating that the supernumerary chromosomes might correspond to distinct complex systems whose origins in the species are still the subject of extensive discussions.

Analyses on the molecular content of amphibian B chromosomes are scarce, and the only reported cases are in the frog Leiopelma hochstetteri Sharbel et al. In both cases, microdissected B chromosomes were analysed by Southern blotting, revealing that the B chromosomes share repetitive sequences with chromosomes of the A complement.

The B chromosome of Leiopelma hochstetteri was also reported to contain specific sequences that were not present in any A chromosomes. Our previous analysis Gruber et al. This supernumerary was very similar in size and morphology to NOR-bearing chromosome 8, and had a large amount of late replicating C-positive heterochromatin, with a small bright DAPI region in the short arms.

It was observed that the B was univalent, not pairing with any other chromosome in metaphase I cells. The present work was carried out to investigate whether the B of Hypsiboas albopunctatus shares sequence homology with chromosomes of the A complement of the same species or of its closely related species Hypsiboas raniceps which could shed light upon questions about its intraspecific or interspecific origin. Direct cytological suspensions of bone marrow, liver, and testes were obtained according to Baldisera Jr.

The specimens of Hypsiboas albopunctatus were injected with 5-bromodoxiuridine BrdU before colchicine treatment Silva et al.

Slides were conventionally stained with Giemsa and those of Hypsiboas albopunctatus with B chromosome were also submitted to FPG Fluorochrome Plus Giemsa technique Dutrillaux and Couturier ; Matsuda and Chapman to differentiate replication banding. The chromosomal images were captured with an Olympus digital camera D71 using the DP Controller program.

Bi-armed chromosomes were classified as metacentric, submetacentric, or subtelocentric Green and Sessions , Microdissections of B chromosome were carried out according to Dinizet al. This procedure was repeated four times for each individual. Thermo Sequenase enzyme USB was added. Finally, a third PCR was performed to label the microdissection products. The amplification steps were the same as described for the previous reaction.

The fragments were — bp. Firstly, the probes were tested on samples from the individuals they were generated from, i. Next, cross-specimen chromosome painting was performed in which the B54 probe was used on mitotic preparations from CFBH Results Fig.

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Kagul Hylidae during the reproductive activity in southeastern Brazil. Seewave, a free modular tool for sound analysis and synthesis. The acoustic behavior of both species was influenced by the number of conspecifics calling at the site, as reported for other anurans e. Species categorized as Near Threatened NT. This is an incredible achievement. White-spotted tree frog Strabomantidae in Central Brazil.

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HYPSIBOAS ALBOPUNCTATUS PDF

Kazinos The most common vocalization of H. The vocal repertoire of both anuran species studied here is hypsjboas of advertisement and aggressive calls, as in other Neotropical species Heyer et al. A language and environment for statistical computing. The former was the most frequent and albopunctatks different structures and functions Table 2.

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Hypsiboas albopunctatus

Comp Cytogenet. Published online Aug 8. This article has been cited by other articles in PMC. Reverse hybridisation was also carried out in the investigation. The B probe exclusively painted the supernumerary, not hybridising any other chromosomes in H. This result might be an indication that differences exist between the repetitive sequences of A and B chromosomes of H. Although our results could suggest an interespecific origin of the B at first sight, further analysis on its repetitive sequences is still necessary.

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